Pig βEP (Beta-Endorphin) ELISA Kit - ELK0332

Pig βEP (Beta-Endorphin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK0332-48, ELK0332-96, ELK0332-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: Beta EP; b-EP

Assay Type: Competitive Inhibition

Sensitivity: 4.95 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: Serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay Length: 2.5h

Research Areas: Signal transduction; Endocrinology; Neuroscience; Hormone metabolism;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Pig βEP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig βEP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig βEP in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only