Pig ADST (Androstenone) ELISA Kit - ELK8586

Pig ADST (Androstenone) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK8586-48, ELK8586-96, ELK8586-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: Androstenone

Assay Type: Competitive Inhibition

Sensitivity: 9.68 nmol/mL

Standard: 2000 nmol/mL

Detection Range: 31.25-2000 nmol/mL

Sample Type: serum, plasma and other biological fluids

Assay Length: 2h

Research Areas: Biochemical research

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Pig ADST. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig ADST. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig ADST in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only