Pig AED (Androstenediol) ELISA Kit - ELK8706

Pig AED (Androstenediol) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK8706-48, ELK8706-96, ELK8706-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: 5-AED; 5AED; 5-Androstenediol; Androst-5-Ene-3Beta,17Beta-Diol

Assay Type: Competitive Inhibition

Sensitivity: 51.8 pg/mL

Standard: 10000 pg/mL

Detection Range: 156.25-10000 pg/mL

Sample Type: serum, plasma and other biological fluids

Assay Length: 2h

Research Areas: Endocrinology; Reproductive science; Hormone metabolism;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Pig AED. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig AED. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig AED in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only