Pig ET-1 (Endothelin1) ELISA Kit - ELK7427

Pig ET-1 (Endothelin1) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK7427-48, ELK7427-96, ELK7427-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: ET1; PPET1; Preproendothelin-1; Big endothelin-1; EDN1

Assay Type: Sandwich

Sensitivity: 4.51 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Length: 3.5h

Research Areas: Endocrinology; Cardiovascular biology;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig ET-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig ET-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig ET-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig ET-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P09558

Research Use Only