Pig FN (Fibronectin) ELISA Kit - ELK11124

Pig FN (Fibronectin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK11124-48, ELK11124-96, ELK11124-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: FN1; CIG; FINC; LETS; MSF; GFND2; Anastellin; Migration-Stimulating Factor; Cold-Insoluble Globulin; Large, External, Transformation-Sensitive Protein

Assay Type: Sandwich

Sensitivity: 1.01 µg/mL

Standard: 100 µg/mL

Detection Range: 1.56-100 µg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Length: 3.5h

Research Areas: Signal transduction; Infection immunity;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig FN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig FN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig FN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig FN in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only