Pig I-PTH (IntactParathormone) ELISA Kit - ELK6409

Pig I-PTH (IntactParathormone) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK6409-48, ELK6409-96, ELK6409-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: iPTH; Intact Parathyroid Hormone; Parathormone; Parathyrin; PTH; Parathyroid Hormone

Assay Type: Sandwich

Sensitivity: 5.59 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Length: 3.5h

Research Areas: Metabolic pathway; Endocrinology; Hormone metabolism;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig I-PTH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig I-PTH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig I-PTH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig I-PTH in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only