Product Description

Pig IL7 (Interleukin 7) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK11389-48, ELK11389-96, ELK11389-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig IL7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig IL7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig IL7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig IL7 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: IL-7, Interleukin-7

Reactivity: Pig

Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatant, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Cytokine, Infection Immunity, Bone Metabolism, Dermatology

Assay Length: 3.5h

Sensitivity: 11.3 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

UniProt: Q9N2G6

Research Use Only