Product Description

Pig OX (Orexin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK11449-48, ELK11449-96, ELK11449-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Pig

Alternate Names: HCRT, PPOX, Orexin A, Hypocretin 1, Orexin B, Hypocretin 2, Preproorexin, Hypocretin Neuropeptide Precursor

Sensitivity: 4.53 pg/mL

Assay Type: Competitive Inhibition

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: Serum, Plasma, Tissue Homogenates, Other Biological Fluids

Assay Length: 2h

Research Area: Neuro Science

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Pig OX. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig OX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig OX in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: O77668

Research Use Only