Pig PAG1 (Pregnancy associated glycoprotein 1) ELISA Kit - ELK8655

Pig PAG1 (Pregnancy associated glycoprotein 1) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK8655-48, ELK8655-96, ELK8655-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: PAG1; Pregnancy associated glycoprotein 1

Assay Type: Sandwich

Sensitivity: 0.056 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: serum, plasma and other biological fluids

Assay Length: 3.5h

Research Areas: Pregnancy diagnosis

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig PAG1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig PAG1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig PAG1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig PAG1 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q29078

Research Use Only