Pig Tie2 (TEK Tyrosine Kinase, Endothelial) ELISA Kit - ELK8471

Pig Tie2 (TEK Tyrosine Kinase, Endothelial) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK8471-48, ELK8471-96, ELK8471-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: CD202b; CD202-B; TEK; VMCM1; Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains 2; Venous Malformations Multiple Cutaneous And Mucosal

Assay Type: Sandwich

Sensitivity: 0.38 ng/mL

Standard: 60 ng/mL

Detection Range: 0.94-60 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Length: 3.5h

Research Areas: Enzyme and Kinase; Tumor immunity; Infection immunity;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig Tie2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig Tie2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig Tie2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig Tie2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only