PKCα (Phospho-T638) Polyclonal Antibody-BS94053

PKCα (Phospho-T638) Polyclonal Antibody Sizes: 50µl, 100µl Catalogue Numbers: BS94053-50, BS94053-100 Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Swiss-Prot: P17252(Human) P20444(Mouse) P05696(Rat) Host: Rabbit Reactivity: Human, Mouse, Rat Applications: WB, ICC/IF, IHC, IP, All Applications: WB:1:1,000-1:5,000 ICC:1:50-1:500 IHC:1:50-1:500 Background: Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor-promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into many different isoforms (α, βI, βII, γ, δ, ε, ζ, η, θ, λ/ι, μ and ν). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of PKC δ and ε are independent of Ca²⁺. On the other hand, most of the other PKC members possess phorbol ester-binding activities and kinase activities. Purification and Purity: ProA affinity purified Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. Specificity: PKCα (Phospho-T638) polyclonal antibody detects endogenous levels of PKCα protein only when phosphorylated at T638. Bioworld Molecular Weight: 77 kDa Note: For research use only, not for use in diagnostic procedure. Extra Notes: Western blot analysis of Phospho-PKC alpha(T638) on different lysates using anti-Phospho-PKC alpha(T638) antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: 293, ICC staining Phospho-PKC alpha(T638) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. Alternative Name: PKC-A, PKC-alpha, PRKCA, AAG6, Aging associated gene 6, aPKC, KPCA, PKC alpha, PKC-A, PKC-alpha, PKCA, PRKACA, PRKCA, Protein Kinase C alpha, Protein kinase C alpha type, Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Thr638 of human PKC alpha. Conjugate: Unconjugated Modification: Phosphorylation