Product Description

PKCε Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91085-50, BS91085-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q02156(Human) P16054(Mouse) P09216(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC IHC FC

All Applications: WB:1:500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: PKCε polyclonal antibody detects endogenous levels of PKCε protein.

Bioworld Molecular Weight: 84 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of PKC epsilon on different cell lysate using anti-PKC epsilon antibody at 1/1,000 dilution.
Positive control:
Lane 1: Jurkat
Lane 2: MCF-7
Lane 3: HT-29, ICC staining PKC epsilon in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: nPKC-epsilon, PRKCE, PKCE, KPCE, MGC125656, MGC125657, nPKC epsilon, nPKC-epsilon, PKCE, Pkcea, PRKCE, Protein kinase C epsilon, Protein kinase C epsilon type,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified