Product Description

PKC α (phospho Tyr658) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES6761-50, ES6761-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq, Jul 2

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC

Alternate Names: PRKCA, PKCA, PRKACA, Protein kinase C alpha type, PKC-A, PKC-alpha

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human PKC alpha around the phosphorylation site of Tyr657. AA range:623-672

Isotype: IgG

Target: PKC α

Specificity: Phospho-PKC α (Y657) Polyclonal Antibody detects endogenous levels of PKC α protein only when phosphorylated at Y657.

Dilution: WB 1:500-2000, IHC-p 1:50-300

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Blood, Brain, Epithelium, Lung, Platelet,

Observed Band (KD): 80kD

Fields: EGFR tyrosine kinase inhibitor resistance, MAPK signaling pathway, ErbB signaling pathway, Ras signaling pathway, Rap1 signaling pathway, Calcium signaling pathway, HIF-1 signaling pathway, Phosphatidylinositol signaling system, Sphingolipid signaling pathway, Phospholipase D signaling pathway, mTOR signaling pathway, PI3K-Akt signaling pathway, Adrenergic signaling in cardiomyocytes, Vascular smooth muscle contraction, Wnt signaling pathway, Axon guidance, VEGF signaling pathway, Focal adhesion, Gap junction, Neutrophil extracellular trap formation, Natural killer cell mediated cytotoxicity, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Leukocyte transendothelial migration, Circadian entrainment, Long-term potentiation, Retrograde endocannabinoid signaling, Glutamatergic synapse, Cholinergic synapse, Serotonergic synapse, GABAergic synapse, Dopaminergic synapse, Long-term depression, Inflammatory mediator regulation of TRP channe

Function: Catalytic Activity: ATP + a protein = ADP + a phosphoprotein
Cofactor: Binds 3 calcium ions per subunit. The ions are bound to the C2 domain
Function: PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters
Function: This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4
Similarity: Belongs to the protein kinase superfamily
Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily
Similarity: Contains 1 AGC-kinase C-terminal domain
Similarity: Contains 1 C2 domain
Similarity: Contains 1 protein kinase domain
Similarity: Contains 2 phorbol-ester/DAG-type zinc fingers
Subunit: Interacts with ADAP1/CENTA1, CSPG4 and PRKCABP. Binds to SDPR

Subcellular Location: Cytoplasm Cell membrane, Peripheral membrane protein Mitochondrion membrane, Peripheral membrane protein Nucleus

Storage and Stability: -20°C/1 year

Gene Name: PRKCA

Protein Name: Protein kinase C alpha type

Mouse Gene ID: 18750

Mouse SwissProt Number: P20444

Rat SwissProt Number: P05696

Research Use Only