Product Description

PKC δ (phospho-S645) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS4622-50, BS4622-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Protein Kinase C delta (PKC delta) is a 78 kDa member of the novel group (nPKCs: sensitive to diacylglycerol, phosphatidylserine, and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regμlation. PKC delta is an ubiquitously expressed PKC isozyme that has been implicated in the regμlation of mμltiple cellμlar processes including cell cycle progression and apoptosis. Autophosphorylation of serine 664 (serine 662 in mouse and rat) contributes to PKC delta activity. Serum dependent phosphorylation of serine 664 is mediated by mTOR pathway, is protected from dephosphorylation by phosphorylated threonine 505 in the activation loop, and is implicated in prolactin signaling.

Category: Primary Antibody

Reactivity: Human

Host: Rabbit

Applications: WB, IHC

Alternate Names: Protein kinase C delta type, Tyrosine-protein kinase PRKCD, nPKC-delta, Sphingosine-dependent protein kinase-1, SDK1, PRKCD, PKCδ

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human PKC δ around the phosphorylation site of Serine 645.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-PKC δ (S645) polyclonal antibody detects endogenous levels of PKC δ protein only when phosphorylated at Ser645.

Molecular Weight: ~ 80 kDa

Pathway: Protein Kinase C Signaling, Inhibition of Apoptosis, Mitochondrial Control of Apoptosis,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q05655

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only