Product Description

PKD1 (phospho Ser205) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES6786-50, ES6786-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: PRKD1 is a serine/threonine kinase that regulates a variety of cellular functions, including membrane receptor signaling, transport at the Golgi, protection from oxidative stress at the mitochondria, gene transcription, and regulation of cell shape, motility, and adhesion (summary by Eiseler et al., 2009 [PubMed 19329994]).[supplied by OMIM, Nov 2010],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: PRKD1, PKD, PKD1, PRKCM, Serine/threonine-protein kinase D1, Protein kinase C mu type, Protein kinase D, nPKC-D1, nPKC-mu

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human PKD1/PKC mu around the phosphorylation site of Ser205. AA range:171-220

Isotype: IgG

Target: PKD1

Specificity: Phospho-PKD1 (S205) Polyclonal Antibody detects endogenous levels of PKD1 protein only when phosphorylated at S205.

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Placenta, Testis,

Observed Band (KD): 110kD

Fields: Rap1 signaling pathway, Aldosterone synthesis and secretion, Chemical carcinogenesis - reactive oxygen species

Function: Catalytic Activity: ATP + a protein = ADP + a phosphoprotein
Enzyme Regulation: Activated by diacylglycerol and phorbol esters
Function: Calcium-independent, phospholipid-dependent, serine- and threonine-specific kinase involved in resistance to oxidative stress
PTM: Phosphorylation of Ser-738 and/or Ser-742 in activated PKD is mediated by transphosphorylation (By similarity). Phosphorylation of Tyr-463 mediated by the Src/Abl pathway in response to oxidative stress activates the kinase
Similarity: Belongs to the protein kinase superfamily
Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily
Similarity: Contains 1 PH domain
Similarity: Contains 1 protein kinase domain
Similarity: Contains 2 phorbol-ester/DAG-type zinc fingers
Subunit: Interacts (via N-terminus) with ADAP1/CENTA1. Interacts with Src

Subcellular Location: Cytoplasm Cell membrane Golgi apparatus, trans-Golgi network Translocation to the cell membrane is required for kinase activation

Storage and Stability: -20°C/1 year

Gene Name: PRKD1

Protein Name: Serine/threonine-protein kinase D1

Mouse Gene ID: 18760

Mouse SwissProt Number: Q62101

Rat Gene ID: 85421

Rat SwissProt Number: Q9WTQ1

Research Use Only