Product Description

PKM1/PKM2 (28-4-11) Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS66500-50, BS66500-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Pyruvate kinase is a glycolytic enzyme that catalyses the conversion of phosphoenolpyruvate to pyruvate. In mammals, the M1 isoform (PKM1) is expressed in most adμlt tissues. The M2 isoform (PKM2) is an alternatively spliced variant of M1 that is expressed during embryonic development. Research studies found that cancer cells exclusively express PKM2. PKM2 is shown to be essential for aerobic glycolysis in tumors, known as the Warburg effect. When cancer cells switch from the M2 isoform to the M1 isoform, aerobic glycolysis is reduced and oxidative phosphorylation is increased. These cells also show decreased tumorigenicity in mouse xenografts. Recent studies showed that PKM2 is not essential for all tumor cells. In the tumor model studied, PKM2 was found to be active in the non-proliferative tumor cell popμlation and inactive in the proliferative tumor cell popμlation.

Category: Primary Antibody

Reactivity: Human, Rat, Mouse

Host: Mouse

Applications: WB, IP

Alternate Names: Pyruvate kinase PKM, Cytosolic thyroid hormone-binding protein, Opa-interacting protein 3, Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, Threonine-protein kinase PKM2, PKM, OIP3, PK2, PK3, PKM2

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodified

Immunogen: Recombinant protein of Human PKM1/PKM2.

Dilution: WB: 1:1000~1:2000
IP: 1:50~1:200

Purification: The antibody was affinity-purified from cell cμlture supernatant by protein A+G and the purity is > 95% (by SDS-PAGE).

Specificity: PKM1/PKM2(28-4-11) polyclonal antibody detects endogenous levels of PKM1/PKM2 protein.

Molecular Weight: ~ 65 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P14618

Product: Mouse IgG, 0.4mg/ml in PBS with 0.05% Proclin300, 50% glycerol, 0.2% BSA, pH7.2.

Research Use Only