Plant GA (Gibberellic Acid) ELISA Kit - ELK8754

Plant GA (Gibberellic Acid) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK8754-48, ELK8754-96, ELK8754-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: GA3; Gibberellin A3; Gibberellins

Assay Type: Competitive Inhibition

Sensitivity: 52 ng/mL

Standard: 10000 ng/mL

Detection Range: 156.25-10000 ng/mL

Sample Type: plant tissues

Assay Length: 2h

Research Areas: Nutrition metabolism; Hormone metabolism;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Plant GA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Plant GA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Plant GA in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only