POLA2 Polyclonal Antibody
Sizes: 60μL, 120μL, 200μL
Catalogue Numbers: E-AB-92532-60, E-AB-92532-120, E-AB-92532-200
Citations, Manuals and MSDS Available upon request.
Abbreviation: POLA2
Target Synonym: POLA2
Conjugation: Unconjugated
Host: Rabbit
Species Reactivity: Human, Mouse, Rat
Application: WB
Isotype: IgG
Clonality: Polyclonal
UNIProt ID: Q14181
Background: Accessory subunit of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex which plays an essential role in the initiation of DNA synthesis. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1, an accessory subunit POLA2 and two primase subunits, the catalytic subunit PRIM1 and the regulatory subunit PRIM2 is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1 (By similarity. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands (By similarity. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively (By similarity.
Concentration: 1mg/mL
Immunogen: Recombinant fusion protein of human POLA2
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification Method: Affinity purification
Dilution: WB 1:1000-1:3000
Calculated MW: 40kDa/65kDa
Observed MW: 70kDa
Storage: -20°C/One year. Avoid freeze / thaw cycles.
Shipping: Ice bag
Research Use Only