PP (Dipalmitin) ELISA Kit - ELK8238

PP (Dipalmitin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK8238-48, ELK8238-96, ELK8238-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: 1,3-Dipalmitin; 1,3-Di- (Hexadecanoyl)-Glycerol; 1,3-Dipalmitoylglycerol; 1,3-Dipalmitoyl-Glycerol, Glyceryl 1,3-Dipalmitate

Assay Type: Competitive Inhibition

Sensitivity: 5.09 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: biological agents

Assay Length: 2h

Research Areas: Enzyme and Kinase

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PP in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only