PP2Aα/β Polyclonal Antibody-BS91098

PP2Aα/β Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91098-50, BS91098-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P62714(Human) P67775(Human) P62715(Mouse) P63330(Mouse) P62716(Rat) P63331(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat, zebrafish

Applications: WB, ICC, IHC, IP,

All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:100-1:200

Background: The catalytic subunit of protein phosphatase 2A (PP2A) is inactivated by in vitro phosphorylation of Tyr-307 by receptor and nonreceptor protein tyrosine kinases. The catalytic subunit of PP2A is phosphorylated by tyrosine-specific protein kinases and associates with a variety of regulatory subunits. Phosphorylation is enhanced in the presence of the phosphatase inhibitor okadaic acid, consistent with an autodephosphorylation reaction. Phosphorylation is catalyzed by p60v-src, p56lck, epidermal growth factor receptors, and insulin receptors. Transient deactivation of PP2A might enhance transmission of cellular signals through kinase cascades within cells. In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including cell division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: PP2Aα/β polyclonal antibody detects endogenous levels of PP2Aα/β protein.

Bioworld Molecular Weight: 36 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of PP2A alpha + beta on different lysates using anti-PP2A alpha + beta antibody at 1/1,000 dilution.
Positive control:
Lane 1: A431
Lane 2: NIH/3T3
Lane 3: 293T, ICC staining PP2A alpha + beta in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: PP2A-alpha, RP-C, PPP2CA, PP2A-beta, PPP2CB, PP2A A, PP2A alpha, PP2A B, PP2A beta, PP2A-alpha, PP2AA, PP2Ac, PP2CB, PPP2CA, PPP2CB, Protein phosphatase 2 catalytic subunit alpha isoform, Protein phosphatase 2 catalytic subunit beta isoform, Replication protein C, RP C, RP-C, Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform, Serine/threonine protein phosphatase 2A catalytic subunit beta isoform, Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified