PP2A-Aα/PR65α/PPP2R1A Polyclonal Antibody-BS7866

PP2A-Aα/PR65α/PPP2R1A Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS7866-50, BS7866-100

Swiss-Prot: P30153

Host: Rabbit

PP2A-Aα/PR65α/PPP2R1A Polyclonal Antibody-BS7866

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:10 - 1:100

Background: This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. Alternatively spliced transcript variants have been described.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 65KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using PP2A-Aalpha/PR65α/PP2A-Aα/PR65α/PPP2R1A Polyclonal Antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s., Immunofluorescence analysis of U2OS cells using PP2A-Aalpha/PR65α/PP2A-Aα/PR65α/PPP2R1A antibody. Blue: DAPI for nuclear staining.

Alternative Name: PPP2R1A; MRD36; PP2A-Aalpha; PP2AA; PP2AAALPHA; PR65A

Immunogen: Recombinant fusion protein of human PP2A-Aalpha/PR65α/PP2A-Aα/PR65α/PPP2R1A(NP_055040.2).

Modification: Unmodified

Conjugate: Unconjugated