Product Description

PPARγ Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS6442-50, BS6442-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P37231

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: IHC, IF/ICC

All Applications: IHC,1:50 - 1:200 | IF/ICC,1:50 - 1:20

Background: This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 57KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry of paraffin-embedded mouse heart using PPARγ Rabbit pAb at dilution of 1:200. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded mouse liver using PPARγ Rabbit pAb at dilution of 1:200. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunofluorescence analysis of U2OS cells using PPARγ Rabbit pAb at dilution of 1:200. Blue: DAPI for nuclear staining.

Alternative Name: CIMT1; GLM1; NR1C3; PPARG1; PPARG2; PPARgamma; PPAR gamma; PPARG

Immunogen: Recombinant fusion protein of human PPARγ(NP_056953.2).

Conjugate: Unconjugated

Modification: Unmodified