Product Description

PRAK Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES7818-50, ES7818-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein encoded by this gene is a tumor suppressor and member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. The encoded protein is found in the nucleus but translocates to the cytoplasm upon phosphorylation and activation. This kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2012],

Reactivity: Human, Mouse

Host: Rabbit

Applications: WB, IHC

Alternate Names: MAPKAPK5, PRAK, MAP kinase-activated protein kinase 5, MAPK-activated protein kinase 5, MAPKAP kinase 5, MAPKAP-K5, MAPKAPK-5, MK-5, MK5, p38-regulated/activated protein kinase, PRAK

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human MAPKAPK5. AA range:148-197

Isotype: IgG

Target: MAPKAPK5

Specificity: PRAK Polyclonal Antibody detects endogenous levels of PRAK protein.

Dilution: WB 1:500-2000, IHC-p 1:50-300

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Expressed ubiquitously,

Observed Band (KD): 60kD

Fields: MAPK signaling pathway

Function: Catalytic Activity: ATP + a protein = ADP + a phosphoprotein
Enzyme Regulation: p38 alpha and beta-dependent phosphorylation increases its activity. Activated by stress-related extracellular stimuli, such as H(2)O(2), arsenite, anisomycin TNF alpha and also PMA and the calcium ionophore A23187, but to a lesser extent. In vitro, activated by SQSTM1
Function: Mediates stress-induced small heat shock protein 27 phosphorylation
PTM: Phosphorylated on Thr-182, which is the regulatory phosphorylation site and is located on the T-loop/loop 12
Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family
Similarity: Contains 1 protein kinase domain
Subcellular Location: Also observed in the nucleus
Subunit: Interacts with SQSTM1
Tissue Specificity: Expressed ubiquitously

Subcellular Location: Cytoplasm Nucleus Translocates to the cytoplasm following phosphorylation and activation Interaction with ERK3/MAPK6 or ERK4/MAPK4 and phosphorylation at Thr-182, activates the protein kinase activity, followed by translocation to the cytoplasm Phosphorylation by PKA/PRKACA at Ser-115 also induces nuclear export

Storage and Stability: -20°C/1 year

Gene Name: MAPKAPK5

Protein Name: MAP kinase-activated protein kinase 5

Mouse Gene ID: 17165

Mouse SwissProt Number: O54992

Research Use Only