Product Description

Prothrombin Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES3856-50, ES3856-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Peptides derived from the C-terminus of this protein have antimicrobial activity against E. coli and P. aeruginosa. Mutations in F2 lead to various forms of thrombosis and dysprothrombinemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: F2, Prothrombin, Coagulation factor II

Clonality: Polyclonal

Immunogen: Synthesized peptide derived from the Internal region of human Prothrombin. AA range 420-470

Isotype: IgG

Target: Prothrombin

Specificity: Prothrombin Polyclonal Antibody detects endogenous levels of Prothrombin protein.

Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Expressed by the liver and secreted in plasma,

Observed Band (KD): 70kD

Fields: Phospholipase D signaling pathway, Neuroactive ligand-receptor interaction, Complement and coagulation cascades, Platelet activation, Regulation of actin cytoskeleton, Pathogenic Escherichia coli infection, Coronavirus disease - COVID-19, Pathways in cancer

Function: Catalytic Activity: Selective cleavage of Arg-|-Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B
Disease: Defects in F2 are the cause of various forms of dysprothrombinemia [MIM: 176930]
Disease: Genetic variations in F2 may be a cause of susceptibility to ischemic stroke [MIM: 601367], also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors
Function: Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protei

Subcellular Location: Secreted, extracellular space

Storage and Stability: -20°C/1 year

Gene Name: F2

Protein Name: Prothrombin

Mouse Gene ID: 14061

Mouse SwissProt Number: P19221

Rat SwissProt Number: P18292

Research Use Only