Product Description

PTRF Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES9010-50, ES9010-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a protein that enables the dissociation of paused ternary polymerase I transcription complexes from the 3' end of pre-rRNA transcripts. This protein regulates rRNA transcription by promoting the dissociation of transcription complexes and the reinitiation of polymerase I on nascent rRNA transcripts. This protein also localizes to caveolae at the plasma membrane and is thought to play a critical role in the formation of caveolae and the stabilization of caveolins. This protein translocates from caveolae to the cytoplasm after insulin stimulation. Caveolae contain truncated forms of this protein and may be the site of phosphorylation-dependent proteolysis. This protein is also thought to modify lipid metabolism and insulin-regulated gene expression. Mutations in this gene result in a disorder characterized by generalized lipodystrophy and muscular dystrop

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, ELISA

Clonality: Polyclonal

Immunogen: Synthesized peptide derived from human protein. at AA range: 90-170

Isotype: IgG

Target: PTRF

Specificity: PTRF Polyclonal Antibody detects endogenous levels of protein.

Dilution: WB 1:500-2000 ELISA 1:5000-20000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Expression: Adipocyte, Epithelium, Lung, Muscle, Pancreas, Testis,

Observed Band (KD): 42kD

Function: Function: Termination of transcription by RNA polymerase I involves pausing of transcription by TTF1, and the dissociation of the transcription complex, releasing pre-rRNA and RNA polymerase I from the template. PTRF is required for dissociation of the ternary transcription complex
PTM: Five truncated forms are found in the caveolae. These are thought to be the result of proteolysis and may be phosphorylation-dependent
PTM: Phosphorylated. Present in active and inactive forms. Changes in phosphorylation pattern may alter activity
Similarity: Belongs to the PTRF/SDPR family
Subcellular Location: Found at the surface of the caveolae. Also found in the plasma membrane, microsomal and cytosolic fractions and at a low level in the mitochondrial and nuclear fractions. Translocates to the cytoplasm from the caveolae upon insulin stimulation
Subunit: Interacts with RNA polymerase I and TTF1. Bind

Subcellular Location: Membrane, caveola Cell membrane Microsome Endoplasmic reticulum Cytoplasm, cytosol Mitochondrion Nucleus Translocates to the cytoplasm from the caveolae upon insulin stimulation (PubMed:17026959) Colocalizes with CAV1 in lipid rafts in adipocytes Localizes in the caveolae in a caveolin-dependent manner (By similarity)

Storage and Stability: -20°C/1 year

Gene Name: PTRF FKSG13

Protein Name: Polymerase I and transcript release factor (Cavin-1)

Mouse SwissProt Number: O54724

Rat SwissProt Number: P85125

Research Use Only