Puromycin Rabbit pAb
Sizes: 20μL, 100μL
Catalogue Numbers: A21205-20, A21205-100
Citations, Manuals and MSDS Available upon request.
Background: Puromycin is a tyrosyl-tRNA mimic that blocks translation by labeling and releasing elongating polypeptide chains from translating ribosomes. Puromycin has been used in molecular biology research for decades as a translation inhibitor.More recently, in vivo puromycylation assays have become popular tools for localizing translating ribosomes in cells. These assays often use elongation inhibitors to purportedly inhibit the release of puromycin-labeled nascent peptides from ribosomes.
Category: Polyclonal Antibodies
Applications: WB, ELISA
Cross-reactivity: Species independent
Observed Molecular Weight: 10-250kDa
Immunogen: Chemical compounds corresponding to Puromycin.
Species: Human
GeneID: CAS:58-58-2
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Recommended Dilutions: WB, 1:1000 - 1:5000 ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
NCBI Alias: Puromycin
Research Use Only