Product Description

Pyruvate Carboxylase (PC) Activity Assay Kit

Sizes: 48T, 96T

Catalogue Numbers: E-BC-K608-M-48, E-BC-K608-M-96

Lead times: 2-3 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: Pyruvate carboxylate (PC) is a biotin-dependent enzyme that converts pyruvate to oxaloacetic acid by carboxylation. PC is widely found in animals, molds and yeasts, but it is absent from plants and most bacteria. In the tricarboxylic acid cycle, it is the main complementary reaction supplying oxoacetate and the first rate-limiting enzyme in the gluconeogenesis process.
The product of the PC-catalyzed substrate reacts with the reducing agent. The reducing agent has a maximum absorbance at 340 nm, and the enzyme activity of PC is calculated by calculating the amount of reducing agent consumed in the reaction.

Abbreviation: PC

Research Areas: Glycolysis and Lipid Metabolism;Energy Metabolism

Result Type: Enzyme activity

Detection Instrument: Microplate reader

Detection Type: Colorimetric method

Detection Range: 6.43-876.53 U/L

Tested Sample Types: Serum;Plasma;Animal tissue;Cell

Assay Length: 35 min

Sensitivity: 6.43 U/L

Inter cv: 3.9-9.7%

Intra cv: 0.6-1.4%

Research Use Only