Product Description

QuicKey Pro Rat IL-1R2 (Interleukin 1 Receptor Type Ⅱ) ELISA Kit

Sizes: 24T, 48T, 96T

Catalogue Numbers: E-OSEL-R0008-24, E-OSEL-R0008-48, E-OSEL-R0008-96

Citations, Manuals and MSDS Available upon request.

Abbreviation: IL-1R2

UNIProt ID: P43303

Target Synonyms: IL1R2; CD121b; CDw121b; IL-1R-2; IL-1RT-2; IL-1RT2; IL1R2c; IL1RB;

Research Areas: Cardiovascular, Immunology, Stem cells

Target Species: Rat

Application: ELISA

Usage: This ELISA kit applies to the in vitro quantitative determination of Rat IL-1R2 concentrations in serum, plasma.

Tested Sample Types: Serum, Plasma

Type: Sandwich

Assay Time: 1.5h

Detection Type: Colormetric

Sensitivity: 18.75 pg/mL

Detection Range: 31.25-2000 pg/mL

Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-1R2. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat IL-1R2 are added to the micro ELISA plate wells. Rat IL-1R2 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Rat IL-1R2.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Rat IL-1R2. The concentration of Rat IL-1R2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Cross Reactivity: This kit recognizes Rat IL-1R2 in samples. No significant cross-reactivity or interference between Rat IL-1R2 and analogues was observed

Recovery Rate: 80%-120%

Precision: Both intra-CV and inter-CV are < 10%.

Storage: 2-8℃, 6 months

Shipping: ice bag