Rabbit ALP (Alkaline Phosphatase) ELISA Kit - ELK5999

Rabbit ALP (Alkaline Phosphatase) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK5999-48, ELK5999-96, ELK5999-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: AKP; ALKP; Basic Phosphatase

Assay Type: Sandwich

Sensitivity: 0.123 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Length: 3.5h

Research Areas: Enzyme and Kinase; Metabolic pathway; Tumor immunity; Infection immunity; Hepatology; Bone metabolism;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit ALP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit ALP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit ALP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit ALP in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only