Rabbit CR (Calretinin) ELISA Kit - ELK9056

Rabbit CR (Calretinin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK9056-48, ELK9056-96, ELK9056-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: CR; Calretinin; CALB2; CAB29; CAL2; 29 kDa calbindin; calbindin 2; calbindin 2; ; 29kD; calretinin; calbindin D29K

Assay Type: Competitive Inhibition

Sensitivity: 0.74 nmol/mL

Standard: 80 nmol/mL

Detection Range: 1.25-80 nmol/mL

Sample Type: Serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay Length: 2h

Research Areas: Tumor immunity; Neuro science

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rabbit CR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit CR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit CR in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only