Rabbit IL8Ra (Interleukin 8 Receptor Alpha) ELISA Kit - ELK2923

Rabbit IL8Ra (Interleukin 8 Receptor Alpha) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK2923-48, ELK2923-96, ELK2923-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: IL-8RA; CD181; CDw128a; CXCR1; CKR1; CMKAR1; IL8R1; IL8RBA; IL8-RA; Chemokine C-X-C motif Receptor 1; High Affinity Interleukin-8 Receptor A

Assay Type: Sandwich

Sensitivity: 0.055 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids.

Assay Length: 3.5h

Research Areas: Signal transduction; CD and Adhesion molecule; Infection immunity;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit IL8Ra. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit IL8Ra. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit IL8Ra, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit IL8Ra in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only