Rabbit Polyclonal Antibody to Human MANF
(Mesencephalic Astrocyte-Derived Neurotrophic Factor)
Size: 100 µg
Catalogue Number: 310-100
Citations, Manuals and MSDS Available upon request.
Immunogen: Human MANF
Immunogen Description: Recombinant human MANF protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. Immunogen is purified from cell culture supernatant
Background: MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson’s disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta
UniProt ID: P55145
Alternative Names: ARMET, ARP
Clonality: Rabbit polyclonal
Class: IgG
Reactivity: Human MANF
Application: ELISA, WB, IF, IHC
Protocol: ELISA 0.1 to 0.2 µg/ml; WB 0.15 to 0.5 µg/ml, IHC 10 µg/ml, IF 1 to 20 µg/ml
No cross-reactivity in MANF-knockout mouse. No cross-reactivity with CDNF in IHC, slightly cross-reacts with purified CDNF in WB.
Polyclonal antibody working titer has to be established practically for each particular antigen and assay format
Purification: MANF-affinity purified
Buffer: Concentrated ammonium sulphate in PBS pH 7.4.
Shipping: This product is shipped in non-frozen liquid form in ambient conditions
Storage: Storage: Store at +4°C upon receipt. As product is (NH4)2SO4 precipitate, mix well by pipetting or vortexing prior use
Research Use Only