Product Description

Rad52 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES3309-50, ES3309-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of this gene is present on chromosome 2. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2014],

Reactivity: Human, Mouse

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: RAD52, DNA repair protein RAD52 homolog

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human RAD52. AA range:70-119

Isotype: IgG

Target: Rad52

Specificity: Rad52 Polyclonal Antibody detects endogenous levels of Rad52 protein.

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Amygdala, Brain, Embryonal rhabdomyosarcoma, Hippocampus, PCR rescued c

Observed Band (KD): 46kD

Fields: Homologous recombination

Function: Function: Involved in double-stranded break repair. Plays a central role in genetic recombination and DNA repair by promoting the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR
Similarity: Belongs to the RAD52 family
Subunit: Forms a undecameric ring

Subcellular Location: Nucleus

Storage and Stability: -20°C/1 year

Gene Name: RAD52

Protein Name: DNA repair protein RAD52 homolog

Mouse Gene ID: 19365

Mouse SwissProt Number: P43352

Research Use Only