Product Description

Rat 3-MH (3-Methylhistidine) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK0251-48, ELK0251-96, ELK0251-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat 3-MH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat 3-MH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat 3-MH in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: 3MH

Reactivity: Rat

Sample Type: Serum, Plasma, other biological fluids

Assay Type: Competitive Inhibition

Research Areas: Metabolism

Assay Length: 3.5h

Sensitivity: 2.71 nmol/mL

Standard: 400 nmol/mL

Detection Range: 6.25-400 nmol/mL

Research Use Only