Rat 6ketoPGF1α (6-keto-PGF1 alpha) ELISA Kit - ELK8363

Rat 6ketoPGF1α (6-keto-PGF1 alpha) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK8363-48, ELK8363-96, ELK8363-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: 6-keto-PGF1 alpha; 6ketoPGF1α

Assay Type: Competitive Inhibition

Sensitivity: 1.4 pg/mL

Standard: 500 pg/mL

Detection Range: 7.8-500 pg/mL

Sample Type: Serum, plasma and other biological fluids

Assay Length: 2h

Research Areas: Neuroscience

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat 6ketoPGF1α. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat 6ketoPGF1α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat 6ketoPGF1α in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only