Rat ACV-A (Activin A) ELISA Kit - ELK0175

Rat ACV-A (Activin A) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK0175-48, ELK0175-96, ELK0175-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: ACVA; Activin Beta A Beta A Homodimer

Assay Type: Sandwich

Sensitivity: 4.57 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: Serum, plasma and other biological fluids

Assay Length: 3.5h

Research Areas: Endocrinology; Reproductive science; Hormone metabolism;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ACV-A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ACV-A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ACV-A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ACV-A in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only