Rat AFM (Afamin) ELISA Kit - ELK11169

Rat AFM (Afamin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK11169-48, ELK11169-96, ELK11169-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: ALB2; ALBA; ALF; A-ALB; Alpha-Albumin

Assay Type: Competitive Inhibition

Sensitivity: 0.89 µg/mL

Standard: 200 µg/mL

Detection Range: 3.13-200 µg/mL

Sample Type: Serum, plasma and other biological fluids

Assay Length: 2h

Research Areas: Tumor immunity; Infection immunity; Hepatology;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat AFM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat AFM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat AFM in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P36953

Research Use Only