Product Description

Rat AFM (Afamin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK11169-48, ELK11169-96, ELK11169-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Rat

Alternate Names: ALB2, ALBA, ALF, A-ALB, Alpha-Albumin

Sensitivity: 0.89 µg/mL

Assay Type: Competitive Inhibition

Standard: 200 µg/mL

Detection Range: 3.13-200 µg/mL

Sample Type: Serum, Plasma, Other Biological Fluids

Assay Length: 2h

Research Area: Tumor Immunity, Infection Immunity, Hepatology

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat AFM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat AFM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat AFM in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P36953

Research Use Only