Rat AP (Aprotinin) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK11283-48, ELK11283-96, ELK11283-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: BPTI; Trasylol; Pancreatic Trypsin Inhibitor; Basic protease inhibitor
Assay Type: Competitive Inhibition
Sensitivity: 0.47 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 2h
Research Areas: Enzyme and Kinase; Cardiovascular biology; Human gene deletion; Hepatology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat AP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat AP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat AP in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only