Product Description

Rat AP (Aprotinin) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK11283-48, ELK11283-96, ELK11283-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat AP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat AP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat AP in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: BPTI, Trasylol, Pancreatic Trypsin Inhibitor, Basic protease inhibitor

Reactivity: Rat

Sample Type: Serum, Plasma, Other Biological Fluids

Assay Type: Competitive Inhibition

Research Areas: Enzyme and Kinase, Cardiovascular Biology, Human Gene Deletion, Hepatology

Assay Length: 2h

Sensitivity: 0.47 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Research Use Only