Rat ATG3 (Autophagy Related Protein 3) ELISA Kit - ELK0556

Rat ATG3 (Autophagy Related Protein 3) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK0556-48, ELK0556-96, ELK0556-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: Autophagy-related protein 3, Preconditioning-inducible gene 1 protein, APG3-like, Apg3l

Assay Type: Sandwich

Sensitivity: 0.15 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: serum, plasma, tissue homogenates and other biological fluids

Assay Length: 3.5h

Research Areas: Metabolic pathway

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ATG3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ATG3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ATG3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ATG3 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q6AZ50

Research Use Only