Rat ATP2A2 (ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2) ELISA Kit
Sizes: 48T, 96T
Catalogue Numbers: ELK7804-48, ELK7804-96
Citations, Manuals and MSDS Available upon request.
Alternative Names: ATP2B; DAR; SERCA2; Calcium pump 2; Endoplasmic reticulum class 1/2 Ca(2+) ATPase; Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.065 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids
Assay length: 3.5h
Research Areas: Signal transduction
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ATP2A2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ATP2A2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ATP2A2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ATP2A2 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P11507
Research Use Only.