Rat bCTx (Beta-Crosslaps) ELISA Kit - ELK1546

Rat bCTx (Beta-Crosslaps) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK1546-48, ELK1546-96, ELK1546-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: Beta CTx; b-CTx; bCTXI; bCTX-I; B-Cr; BCL; Type I Collagen C-Telopeptide-Related Fraction

Assay Type: Competitive Inhibition

Sensitivity: 39.1 pg/mL

Standard: 8000 pg/mL

Detection Range: 125-8000 pg/mL

Sample Type: serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay Length: 2h

Research Areas: Bone metabolism;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat bCTx. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat bCTx. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat bCTx in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only