Product Description

Rat BMG/b2-MG (Beta-2-Microglobulin) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK10610-48, ELK10610-96, ELK10610-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat BMG/b2-MG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat BMG/b2-MG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat BMG/b2-MG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat BMG/b2-MG in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: Beta 2M, BMG, B2MG, b2M, Beta-2-Microglobulin

Reactivity: Rat

Sample Type: Serum, Plasma, urine, Tissue Homogenates, Cell Lysates, Cell Culture Supernatant, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Infection Immunity, Kidney Biomarker

Assay Length: 3.5h

Sensitivity: 0.11 µg/mL

Standard: 20 µg/mL

Detection Range: 0.32-20 µg/mL

UniProt: P07151

Research Use Only