Product Description

Rat BMP7 (Bone Morphogenetic Protein 7) Microsample ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK2636MS-48, ELK2636MS-96, ELK2636MS-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat BMP7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat BMP7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat BMP7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat BMP7 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternate Names: OP1, Osteogenic Protein-1, Eptotermin alfa

Reactivity: Rat

Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatant, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Metabolic Pathway, Developmental Science, Bone Metabolism

Assay Length: 3.5h

Sensitivity: 5.2 pg/mL

Standard: 1000 pg/mL

Detection Method: 15.63-1000 pg/mL

Research Use Only