Product Description

Rat CD40L (Cluster Of Differentiation 40 Ligand) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK2526-48, ELK2526-96, ELK2526-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CD40L. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CD40L. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CD40L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CD40L in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: CD154, TRAP, HIGM1, IGM, IMD3, TBAM, T-BAM, TNFSF5, Gp39, TNF Superfamily Member 5, Hyper-IgM Syndrome, TNF-Related Activation Protein, T-Cell B-Cell Activating Molecule

Reactivity: Rat

Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Signal Transduction, Cd and Adhesion Molecule, Apoptosis, Tumor Immunity, Infection Immunity, Immune Molecule

Assay Length: 3.5h

Sensitivity: 0.052 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

UniProt: Q9Z2V2

Research Use Only