Rat CDK2 (Cyclin Dependent Kinase 2) ELISA Kit - ELK6998

Rat CDK2 (Cyclin Dependent Kinase 2) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK6998-48, ELK6998-96, ELK6998-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: CDKN2; p33 (CDK2); Cell division protein kinase 2; p33 protein kinase

Assay Type: Sandwich

Sensitivity: 0.135 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Length: 3.5h

Research Areas: Enzyme and Kinase; Metabolic pathway; Developmental science;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CDK2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CDK2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CDK2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CDK2 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q63699

Research Use Only