Product Description

Rat CDK5 (Cyclin Dependent Kinase 5) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK4419-48, ELK4419-96, ELK4419-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CDK5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CDK5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CDK5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CDK5 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: PSSALRE, CDKN5, Cell division protein kinase 5, Serine/threonine-protein kinase PSSALRE, Tau protein kinase II catalytic subunit, TPKII catalytic subunit

Reactivity: Rat

Sample Type: Tissue Homogenates, Cell Lysates, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Signal Transduction, Enzyme and Kinase, Neuro Science, Developmental Science

Assay Length: 3.5h

Sensitivity: 0.052 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

UniProt: Q03114

Research Use Only