Product Description

Rat COL3 (Collagen Type III) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK2571-48, ELK2571-96, ELK2571-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Rat

Alternate Names: Collagen Type 3, Col 3, Collagen Type III, Col III

Sensitivity: 0.126 ng/mL

Assay Type: Sandwich

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Serum, Plasma, Tissue Homogenates, Other Biological Fluids

Assay Length: 3.5h

Research Area: Metabolic Pathway, Cardiovascular Biology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat COL3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat COL3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat COL3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat COL3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only