Product Description

Rat Cot (Cotinine) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK11825-48, ELK11825-96, ELK11825-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Rat

Alternate Names: Cotinine

Sensitivity: 0.63 ng/mL

Assay Type: Competitive Inhibition

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates, Other Biological Fluids

Assay Length: 2.5h

Research Area: Signal Transduction

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat Cot. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Cot. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Cot in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only