Rat CRAT (Carnitine Acetyltransferase) ELISA Kit - ELK7183

Rat CRAT (Carnitine Acetyltransferase) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK7183-48, ELK7183-96, ELK7183-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: CAT1; Carnitine O-Acetyltransferase; Carnitine acetylase

Assay Type: Sandwich

Sensitivity: 1.27 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Length: 3.5h

Research Areas: Enzyme and Kinase; Metabolic pathway;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat C. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat C in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q704S8

Research Use Only