Rat CUBN (Cubilin) ELISA Kit - ELK7401

Rat CUBN (Cubilin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK7401-48, ELK7401-96, ELK7401-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: IFCR; MGA1; gp280; Intrinsic Factor Cobalamin Receptor; 460 kDa receptor; Intestinal intrinsic factor receptor; Intrinsic factor-vitamin B12 receptor

Assay Type: Sandwich

Sensitivity: 0.11 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Length: 3.5h

Research Areas: Signal transduction; Metabolic pathway; Nutrition metabolism; Dermatology;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CUBN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CUBN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CUBN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CUBN in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: O70244

Research Use Only