Product Description

Rat CUBN (Cubilin) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK7401-48, ELK7401-96, ELK7401-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CUBN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CUBN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CUBN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CUBN in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: IFCR, MGA1, gp280, Intrinsic Factor Cobalamin Receptor, 460 kDa receptor, Intestinal intrinsic factor receptor, Intrinsic factor-vitamin B12 receptor

Reactivity: Rat

Sample Type: Tissue Homogenates, Cell Lysates, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Signal Transduction, Metabolic Pathway, Nutrition Metabolism, Dermatology

Assay Length: 3.5h

Sensitivity: 0.11 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

UniProt: O70244

Research Use Only